FlowJo is currently developed by the Ashland, Oregon-based FlowJo LLC, a subsidiary of Becton Dickinson. In 2002, Tree Star released a Windows version. calcium flux analysis, proliferation analysis, quantification, cluster identification and backgating displayįlowJo became a commercial product in 1996. Thus, you can apply a gate to a sample, copy it to the group, and that gate will be automatically placed on all samples in the group.įlowJo provides tools for the creation of histogram and other plot overlays, cell cycle analysis. When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group. The Windows 7.6.5 workspace support is new and dont support every feature yet - ellipse gates for example. Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came. Good news We support the Mac 9.4.10 and Windows 7.6.5 workspaces in the upcoming 12.1 release. FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations. The node is added on to the destination node as a 'child' of the destination (i.e., it is applied to only those cells. Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. Copying analyses (gates, statistics, kinetics, cell cycle, etc.) from one sample to another is very easy: simply click on the analysis (a gate, statistic, or population node), and drag it to the destination. In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics).
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